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ProductsBack/CASP3 Antibody (Cleaved-Asp175) (OASG01030)

CASP3 Antibody (Cleaved-Asp175) (OASG01030)

OASG01030
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetCasp3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CASP3 Antibody (Cleaved-Asp175) (OASG01030)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID12367
  • Target name
    Casp3
  • Target description
    caspase 3
  • Target synonyms
    A830040C14Rik, AC-3, CASP-3, CC3, CPP-32, CPP32, Caspase-3, Lice, SCA-1, Yama, mldy, caspase-3, SREBP cleavage activity 1, apopain, caspase 3, apoptosis related cysteine protease, cysteine protease CPP32, protein Yama
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-3 (P17) using anti-Caspase-3 (P17) antibody (PA1961-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Heart Tissue Lysate Lane 2: Rat Liver Tissue Lysate Lane 3: Rat Thymus Tissue Lysate Lane 4: Rat Spleen Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 (P17) antigen affinity purified polyclonal antibody (Catalog # PA1961-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 (P17) at approximately 32KD,19KD. The expected band size for Caspase-3 (P17) is at 32KD,17KD.
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