Western Blot Positive WB detected in: Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue All lanes: CASP4 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 37, 14, 31, 19 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue All lanes: CASP4 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 37, 14, 31, 19 kDa Observed band size: 44 kDa
CASP4 Antibody
CSB-PA004549LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCASP4
Overview
- SupplierCusabio
- Product NameCASP4 Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID837
- Target nameCASP4
- Target descriptioncaspase 4
- Target synonymsapoptotic cysteine protease Mih1/TX; CASP-4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; caspase-4; caspase-4 isoform alpha; ICE and Ced-3 homolog 2; ICE(rel)II; ICE(rel)-II; ICEREL-II; ICH-2; Mih1; Mih1/TX; protease ICH-2; protease TX; TX
- HostRabbit
- IsotypeIgG
- Protein IDP49662
- Protein NameCaspase-4
- Scientific DescriptionInflammatory caspase (PubMed:7797510, PubMed:23516580, PubMed:25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer\\\s patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:7743998, PubMed:7797592, PubMed:7797510).
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203