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CASP5 / Caspase 5 Antibody

LS-C816032
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCASP5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CASP5 / Caspase 5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:50 - 1:200) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID838
  • Target name
    CASP5
  • Target description
    caspase 5
  • Target synonyms
    caspase 5 variant X1; caspase 5, apoptosis-related cysteine peptidase; caspase 5, apoptosis-related cysteine protease; caspase-5; ICE(rel)III; ICEREL-III; ICH-3; protease ICH-3; protease TY; TY protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-5/CASP5 using anti-Caspase-5/CASP5 antibody (A05259-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-5/CASP5 antigen affinity purified polyclonal antibody (Catalog # A05259-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-5/CASP5 at approximately 50KD. The expected band size for Caspase-5/CASP5 is at 50KD.
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