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Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CASP9 Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CASP9 Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CASP9 Polyclonal Antibody at dilution 1:60

CASP9 Polyclonal Antibody

E-AB-10054
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CASP9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-9 p35/Casp9 using anti-Caspase-9 p35/Casp9 antibody (A00080-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9 p35/Casp9 antigen affinity purified polyclonal antibody (Catalog # A00080-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-9 p35/Casp9 at approximately 37/46KD. The expected band size for Caspase-9 p35/Casp9 is at 37/46KD.
Product group Antibodies
Boster Bio
Anti-Caspase-9 p35/Casp9 Antibody Picoband(r)A00080-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetCasp9
  • SizePrice