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Western Blot analysis of various samples using CASP9 Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using CASP9 Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various samples using CASP9 Polyclonal Antibody at dilution of 1:1000.

CASP9 Polyclonal Antibody

E-AB-70332
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CASP9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Caspase 9,apoptosis-related cysteine protease (CASP9,synonyms: MCH6,APAF3,APAF-3,ICE-LAP6,CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits,large and small,that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-9 p35/Casp9 using anti-Caspase-9 p35/Casp9 antibody (A00080-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9 p35/Casp9 antigen affinity purified polyclonal antibody (Catalog # A00080-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-9 p35/Casp9 at approximately 37/46KD. The expected band size for Caspase-9 p35/Casp9 is at 37/46KD.
Product group Antibodies
Boster Bio
Anti-Caspase-9 p35/Casp9 Antibody Picoband(r)A00080-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse, Rat
TargetCasp9
  • SizePrice