Mouse Caspase-1 is detected in cell culture supernatants by ELISA using Caspase-1 (mouse) Matched Pair Detection Set (Prod. No AG-46B-0003). Method: Caspase-1 (mouse) was measured by ELISA in supernatants of differentiated bone marr
Caspase-1 (mouse) Matched Pair Detection Set
AG-46B-0003
Overview
- SupplierAdipoGen Life Sciences
- Product NameCaspase-1 (mouse) Matched Pair Detection Set
- Delivery Days Customer10
- ApplicationsELISA
- Assay Detection Range0.15ng/ml to 10ng/ml.
- Assay Sensitivity50pg/ml
- Assay SpecificityDetects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1.
- CertificationResearch Use Only
- Scientific DescriptionCaspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion. - Matched Pair Detection Set. Detects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1. Colorimetric assay. Sample Type: Cell Culture Supernatant. Range: 0.15ng/ml to 10ng/ml. Sensitivity: 50pg/ml. Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
- ReactivityMouse
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC41116133