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Western blot analysis of Caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody (GTX22012) at 1microg/ml.
Western blot analysis of Caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody (GTX22012) at 1microg/ml.
Western blot analysis of Caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody (GTX22012) at 1microg/ml.

Caspase 10 antibody

GTX22012
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetCASP10
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Overview

  • Supplier
    GeneTex
  • Product Name
    Caspase 10 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID843
  • Target name
    CASP10
  • Target description
    caspase 10
  • Target synonyms
    ALPS2; apoptotic protease MCH-4; CASP-10; caspase 10 apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; caspase-10; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; FLICE-2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; MCH4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92851
  • Protein Name
    Caspase-10
  • Scientific Description
    This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-10/CASP10 using anti-Caspase-10/CASP10 antibody (A02190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-10/CASP10 antigen affinity purified polyclonal antibody (Catalog # A02190-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-10/CASP10 at approximately 59 kDa. The expected band size for Caspase-10/CASP10 is at 59 kDa.
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