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caspase-11 (17D9)

SC-56038
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityMouse, Rat
TargetCasp4
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    caspase-11 (17D9)
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID12363
  • Target name
    Casp4
  • Target description
    caspase 4, apoptosis-related cysteine peptidase
  • Target synonyms
    CASP-11, CASP-4, Casp11, Caspl, ich-3, caspase-4, caspase 11, apoptosis-related cysteine peptidase, caspase 11, apoptosis-related cysteine protease, caspase 4, apoptosis-related cysteine protease, caspase-11 short form splicing, protease ICH-3
  • Host
    Rat
  • Isotype
    IgG2a
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates, Lane 6: mouse NIH3T3 whole cell lysates, Lane 7: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 4 antigen affinity purified polyclonal antibody (Catalog # A02941-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 4 at approximately 43KD. The expected band size for Caspase 4 is at 43KD.
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