Caspase-3 Assay Kit (Fluorometric)

LifeSpan BioSciences

Caspase-3 Assay Kit (Fluorometric)

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For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors.

Spectrophotometry (fluorometric) For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors.

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Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction. This Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product.The fluorescence intensity (lambda ex/em = 400/490nm) is proportional to the caspase-3 activity.

-20°C

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