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IHC-P analysis of rat skeletal muscle tissue using GTX31705 Caspase 7 antibody. Working concentration : 10 microg/ml
IHC-P analysis of rat skeletal muscle tissue using GTX31705 Caspase 7 antibody. Working concentration : 10 microg/ml
IHC-P analysis of rat skeletal muscle tissue using GTX31705 Caspase 7 antibody. Working concentration : 10 microg/ml

Caspase 7 antibody

GTX31705
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCASP7
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Overview

  • Supplier
    GeneTex
  • Product Name
    Caspase 7 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 - 1 microg/mL. IHC-P: 10 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID840
  • Target name
    CASP7
  • Target description
    caspase 7
  • Target synonyms
    apoptotic protease MCH-3; CASP-7; caspase 7, apoptosis-related cysteine peptidase; caspase 7, apoptosis-related cysteine protease; caspase-7; CMH-1; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55210
  • Protein Name
    Caspase-7
  • Scientific Description
    This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-7/CASP7 using anti-Caspase-7/CASP7 antibody (A01044-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human T47D whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-7/CASP7 antigen affinity purified polyclonal antibody (Catalog # A01044-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-7/CASP7 at approximately 35KD. The expected band size for Caspase-7/CASP7 is at 35KD.
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Anti-Caspase-7/CASP7 Antibody Picoband(r)A01044-2-CARRIER-FREE
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