![Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 antibody [N2C3] (GTX112888) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 antibody [N2C3] (GTX112888) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112888/GTX112888_42928_20220826_WB_treatment_cisplatin_22083119_157.webp)
Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 antibody [N2C3] (GTX112888) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Caspase 9 antibody [N2C3]
GTX112888
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetCASP9
Overview
- SupplierGeneTex
- Product NameCaspase 9 antibody [N2C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.55 mg/ml
- ConjugateUnconjugated
- Gene ID842
- Target nameCASP9
- Target descriptioncaspase 9
- Target synonymsAPAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, caspase-9, ICE-like apoptotic protease 6, apoptotic protease MCH-6, apoptotic protease activating factor 3, caspase 9, apoptosis-related cysteine peptidase, protein phosphatase 1, regulatory subunit 56
- HostRabbit
- IsotypeIgG
- Protein IDP55211
- Protein NameCaspase-9
- Scientific DescriptionThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq]
- ReactivityCanine, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![Caspase 9 antibody [N2C3] detects Caspase 9 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Caspase 9 stained by Caspase 9 antibody [N2C3] (GTX112888) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Caspase 9 antibody [N2C3] detects Caspase 9 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Caspase 9 stained by Caspase 9 antibody [N2C3] (GTX112888) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112888/GTX112888_42928_20200221_IHC-P_w_23060500_292.webp)









