ELISA of Purified Goat Anti-Cat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Cat IgG. Each well was coated in duplicate with 10 µg of Cat IgG (p/n 002-0102). The working dilution of Cat IgG Alk Phos is 1:2000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and NPP Substrate (p/n NPP-100).