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WB analysis of mouse liver tissue lysate using GTX32491 Cathepsin G antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse liver tissue lysate using GTX32491 Cathepsin G antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse liver tissue lysate using GTX32491 Cathepsin G antibody. Dilution : 1:1000 Loading : 25microg per lane

Cathepsin G antibody

GTX32491
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCTSG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cathepsin G antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1511
  • Target name
    CTSG
  • Target description
    cathepsin G
  • Target synonyms
    CATG; cathepsin G; CG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08311
  • Protein Name
    Cathepsin G
  • Scientific Description
    The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cathepsin G/CTSG using anti-Cathepsin G/CTSG antibody (PB10053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G/CTSG antigen affinity purified polyclonal antibody (Catalog # PB10053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin G/CTSG at approximately 29 kDa. The expected band size for Cathepsin G/CTSG is at 29 kDa.
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