Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Cav2.2 antibody [C3], C-term (GTX102449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Cav2.2 antibody [C3], C-term detects Cav2.2 protein at cell membrane and nucleus by immunofluorescent analysis. Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cav2.2 stained by Cav2.2 antibody [C3], C-term (GTX102449) diluted at 1:500. Red: NF-H, an axon marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX102449/GTX102449_44426_20230811_ICC_IF_M_23090619_312.webp "Cav2.2 antibody [C3], C-term detects Cav2.2 protein at cell membrane and nucleus by immunofluorescent analysis. Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cav2.2 stained by Cav2.2 antibody [C3], C-term (GTX102449) diluted at 1:500. Red: NF-H, an axon marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Cav2.2 antibody [C3], C-term (GTX102449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cav2.2 antibody [C3], C-term
GTX102449
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCACNA1B
Overview
- SupplierGeneTex
- Product NameCav2.2 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.37 mg/ml
- ConjugateUnconjugated
- Gene ID774
- Target nameCACNA1B
- Target descriptioncalcium voltage-gated channel subunit alpha1 B
- Target synonymsBIII, CACNL1A5, CACNN, Cav2.2, DYT23, NEDNEH, voltage-dependent N-type calcium channel subunit alpha-1B, Cav2.2 voltage-gated Ca2+ channel, brain calcium channel III, calcium channel alpha12.2 subunit, calcium channel, L type, alpha-1 polypeptide, calcium channel, voltage-dependent, L type, alpha 1B subunit, calcium channel, voltage-dependent, N type, alpha 1B subunit, voltage-gated calcium channel alpha subunit Cav2.2, voltage-gated calcium channel subunit alpha Cav2.2
- HostRabbit
- IsotypeIgG
- Protein IDQ00975
- Protein NameVoltage-dependent N-type calcium channel subunit alpha-1B
- Scientific DescriptionThe protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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