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Caveolin 2 antibody

GTX108294
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCAV2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Caveolin 2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.94 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID858
  • Target name
    CAV2
  • Target description
    caveolin 2
  • Target synonyms
    CAV; caveolae protein, 20-kD; caveolin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51636
  • Protein Name
    Caveolin-2
  • Scientific Description
    The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Two transcript variants encoding distinct isoforms have been identified for this gene. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by one transcript. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Interaction of Caveolin-3 and HCN is involved in the pathogenesis of diabetic cystopathy. Dong X et al., 2016 Apr 28, Sci Rep
    Read more

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Figure 1. Western blot analysis of Caveolin-2/CAV2 using anti-Caveolin-2/CAV2 antibody (A01574). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: monkey COS-7 whole cell lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: mouse skeletal muscle tissue lysates, Lane 12: mouse heart tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-2/CAV2 antigen affinity purified polyclonal antibody (Catalog # A01574) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-2/CAV2 at approximately 22KD. The expected band size for Caveolin-2/CAV2 is at 22KD.
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