GeneTex anti-hPC2 antibody (GTX24189) was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Polyethylene glycol tert-octylphenyl ether-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37o C. Nuclei were counterstained with Hoechst stain (blue).
GeneTex anti-hPC2 antibody (GTX24189) was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Polyethylene glycol tert-octylphenyl ether-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37o C. Nuclei were counterstained with Hoechst stain (blue).
CBX4 antibody
GTX24189
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCBX4
Overview
- SupplierGeneTex
- Product NameCBX4 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:4000. ICC/IF: 1:100-1:400. IP: 1:100. ELISA: 1:10000-1:40000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.36 mg/ml
- ConjugateUnconjugated
- Gene ID8535
- Target nameCBX4
- Target descriptionchromobox 4
- Target synonymschromobox homolog 4 (Pc class homolog, Drosophila); chromobox protein homolog 4; chromobox-like protein 4; E3 SUMO-protein ligase CBX4; NBP16; NS5ATP1-binding protein 16; Pc class 2 homolog; PC2; polycomb 2 homolog
- HostRabbit
- IsotypeIgG
- Protein IDO00257
- Protein NameE3 SUMO-protein ligase CBX4
- Scientific DescriptionPC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203