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ProductsBack/CCBP2 Antibody - N-terminal region (ARP30776_P050)

CCBP2 Antibody - N-terminal region (ARP30776_P050)

ARP30776_P050
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetACKR2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CCBP2 Antibody - N-terminal region (ARP30776_P050)
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID1238
  • Target name
    ACKR2
  • Target description
    atypical chemokine receptor 2
  • Target synonyms
    CCBP2, CCR10, CCR9, CMKBR9, D6, hD6, atypical chemokine receptor 2, C-C chemokine receptor D6, CC-chemokine-binding receptor JAB61, chemokine (C-C motif) receptor 9, chemokine (C-C) receptor 9, chemokine receptor CCR-10, chemokine receptor CCR-9, chemokine receptor D6, chemokine-binding protein 2, chemokine-binding protein D6
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACKR2 using anti-ACKR2 antibody (A05491-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACKR2 antigen affinity purified polyclonal antibody (Catalog # A05491-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACKR2 at approximately 55KD. The expected band size for ACKR2 is at 43KD.
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