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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CCDC6 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CCDC6 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CCDC6 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa

CCDC6 Antibody

CSB-PA623068LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCCDC6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCDC6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8030
  • Target name
    CCDC6
  • Target description
    coiled-coil domain containing 6
  • Target synonyms
    coiled-coil domain-containing protein 6; D10S170; H4; papillary thyroid carcinoma-encoded protein; PTC; TPC; TST1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16204
  • Protein Name
    Coiled-coil domain-containing protein 6
  • Scientific Description
    cytosol, structural constituent of cytoskeleton
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCDC6 using anti-CCDC6 antibody (PB10050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC6 antigen affinity purified polyclonal antibody (Catalog # PB10050) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC6 at approximately 66 kDa. The expected band size for CCDC6 is at 53 kDa.
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