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ProductsBack/CCL13 / MCP4 Antibody

CCL13 / MCP4 Antibody

LS-C765167
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCCL13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCL13 / MCP4 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:2000 - 1:10000), IHC (1:30 - 1:150) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6357
  • Target name
    CCL13
  • Target description
    C-C motif chemokine ligand 13
  • Target synonyms
    CKb10, MCP-4, NCC-1, NCC1, SCYA13, SCYL1, C-C motif chemokine 13, CK-beta-10, chemokine (C-C motif) ligand 13, monocyte chemoattractant protein 4, monocyte chemotactic protein 4, new CC chemokine 1, small inducible cytokine subfamily A (Cys-Cys), member 13, small-inducible cytokine A13
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCP4 using anti-MCP4 antibody (PB9030). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human MCP4 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP4 antigen affinity purified polyclonal antibody (Catalog # PB9030) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP4 at approximately 34KD. The expected band size for MCP4 is at 34KD.
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