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Western Blot analysis of HL-60 22RV-1 cells using MIP-1beta Polyclonal Antibody
Western Blot analysis of HL-60 22RV-1 cells using MIP-1beta Polyclonal Antibody
Western Blot analysis of HL-60 22RV-1 cells using MIP-1beta Polyclonal Antibody

CCL4 Antibody

CSB-PA005136
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCCL4
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCL4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6351
  • Target name
    CCL4
  • Target description
    C-C motif chemokine ligand 4
  • Target synonyms
    ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, MIP-1-beta(1-69), PAT 744, SIS-gamma, T-cell activation protein 2, chemokine (C-C motif) ligand 4, lymphocyte activation gene 1 protein, macrophage inflammatory protein 1-beta, secreted protein G-26, small inducible cytokine A4 (homologous to mouse Mip-1b)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13236
  • Protein Name
    C-C motif chemokine 4
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCL4 using anti-CCL4 antibody (PB9572). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL4 antigen affinity purified polyclonal antibody (Catalog # PB9572) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL4 at approximately 11 kDa. The expected band size for CCL4 is at 11 kDa.
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