Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody [HL2726] (GTX639554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![CCN3 antibody [HL2726] detects secreted CCN3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. CCN3 stained by CCN3 antibody [HL2726] (GTX639554) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639554/GTX639554_T-45264_20240126_IHC-P_R_24021917_441.webp "CCN3 antibody [HL2726] detects secreted CCN3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. CCN3 stained by CCN3 antibody [HL2726] (GTX639554) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CCN3 antibody [HL2726] detects CCN3 protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CCN3 stained by CCN3 antibody [HL2726] (GTX639554) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639554/GTX639554_T-45264_20240202_ICC_IF_24021917_684.webp "CCN3 antibody [HL2726] detects CCN3 protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CCN3 stained by CCN3 antibody [HL2726] (GTX639554) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody [HL2726] (GTX639554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639554/GTX639554_45341_20240308_WB_TPM_watermark_24031220_201.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody [HL2726] (GTX639554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CCN3 antibody [HL2726] (GTX639554) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639554/GTX639554_T-45264_20240927_WB_B_competitor_watermark_24100318_231.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CCN3 antibody [HL2726] (GTX639554) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody [HL2726] (GTX639554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CCN3 antibody [HL2726]
GTX639554
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetCCN3
Overview
- SupplierGeneTex
- Product NameCCN3 antibody [HL2726]
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2726
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4856
- Target nameCCN3
- Target descriptioncellular communication network factor 3
- Target synonymsIBP-9, IGFBP-9, IGFBP9, NOV, NOVh, CCN family member 3, IGF-binding protein 9, insulin-like growth factor-binding protein 9, nephro blastoma-overexpressed gene protein homolog, nephroblastoma overexpressed, nephroblastoma-overexpressed gene protein homolog, protein NOV homolog
- HostRabbit
- IsotypeIgG
- Protein IDP48745
- Protein NameCCN family member 3
- Scientific DescriptionThe protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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