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ProductsBack/CCR10 Antibody - N-terminal region

CCR10 Antibody - N-terminal region

OAAB10044
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCCR10
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CCR10 Antibody - N-terminal region
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: 1:1000
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID2826
  • Target name
    CCR10
  • Target description
    C-C motif chemokine receptor 10
  • Target synonyms
    GPR2, C-C chemokine receptor type 10, C-C CKR-10, CC chemokine receptor 10, CC-CKR-10, CCR-10, G-protein coupled receptor 2, chemokine (C-C motif) receptor 10
  • Host
    Rabbit
  • Protein IDP46092
  • Protein Name
    C-C chemokine receptor type 10
  • Scientific Description
    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCR10 using anti-CCR10 antibody (A04731-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR10 antigen affinity purified polyclonal antibody (Catalog # A04731-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR10 at approximately 45KD. The expected band size for CCR10 is at 38KD.
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