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Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CCR5/CD195 Polyclonal Antibody, Unconjugated (bs-2514R) followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CCR5/CD195 Polyclonal Antibody, Unconjugated (bs-2514R) followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CCR5/CD195 Polyclonal Antibody, Unconjugated (bs-2514R) followed by conjugation to the secondary antibody

CCR5 Polyclonal Antibody

BS-2514R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCR5
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Overview

  • Supplier
    Bioss
  • Product Name
    CCR5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1234
  • Target name
    CCR5
  • Target description
    C-C motif chemokine receptor 5
  • Target synonyms
    CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22, C-C chemokine receptor type 5, C-C motif chemokine receptor 5 A159A, HIV-1 fusion coreceptor, chemokine (C-C motif) receptor 5, chemokine receptor CCR5, chemokine recptor CCR5 Delta32, chemr13, mutant C-C motif chemokine receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51681
  • Protein Name
    C-C chemokine receptor type 5
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CCR5 using anti-CCR5 antibody (A00061-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR5 antigen affinity purified polyclonal antibody (Catalog # A00061-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR5 at approximately 40KD. The expected band size for CCR5 is at 40KD.
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