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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes: CCT5 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 50 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes: CCT5 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 50 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes: CCT5 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 50 kDa Observed band size: 60 kDa

CCT5 Antibody

CSB-PA004862EA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetCCT5
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCT5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22948
  • Target name
    CCT5
  • Target description
    chaperonin containing TCP1 subunit 5
  • Target synonyms
    CCTE; CCT-epsilon; chaperonin containing TCP1, subunit 5 (epsilon); epididymis secretory protein Li 69; HEL-S-69; PNAS-102; T-complex protein 1 subunit epsilon; TCP-1-epsilon
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48643
  • Protein Name
    T-complex protein 1 subunit epsilon
  • Scientific Description
    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCT5 using anti-CCT5 antibody (PB9928). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U20S whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse testis tissue lysates, Lane 11: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody (Catalog # PB9928) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT5 at approximately 60 kDa. The expected band size for CCT5 is at 60 kDa.
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