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CCT8 Antibody

LS-C408770
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCT8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCT8 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 51kDa/57kDa/59kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 51kDa/57kDa/59kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10694
  • Target name
    CCT8
  • Target description
    chaperonin containing TCP1 subunit 8
  • Target synonyms
    C21orf112, Cctq, D21S246, PRED71, T-complex protein 1 subunit theta, CCT-theta, TCP-1-theta, chaperonin containing T-complex polypeptide 1 subunit 8, chaperonin containing TCP1, subunit 8 (theta), renal carcinoma antigen NY-REN-15
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Western Blot Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate All lanes: CCT8 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 58, 52 kDa Observed band size: 60 kDa
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 theta antibody [N2C2], Internal (GTX105725) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of CCT8 using anti-CCT8 antibody (PB9993). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT8 antigen affinity purified polyclonal antibody (Catalog # PB9993) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT8 at approximately 60 kDa. The expected band size for CCT8 is at 60 kDa.
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