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ProductsBack/CD119 / IFNGR1 Antibody (aa181-193)

CD119 / IFNGR1 Antibody (aa181-193)

LS-C186524
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIFNGR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD119 / IFNGR1 Antibody (aa181-193)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in lysates of cell line HepG2 (calculated MW of 54.4kD according to NP_000407.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 ug/ml.. Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in lysates of cell line HepG2 (calculated MW of 54.4kD according to NP_000407.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3459
  • Target name
    IFNGR1
  • Target description
    interferon gamma receptor 1
  • Target synonyms
    antiviral protein, type 2; AVP, type 2; CD119; CD119 antigen; CDw119; IFN-gamma receptor 1; IFN-gamma-R1; IFN-gamma-R-alpha; IFNGR; IMD27A; IMD27B; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFNGR1 using anti-IFNGR1 antibody (A01716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HEPG2 whole cell lysates, lane 2: SKOV3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNGR1 antigen affinity purified polyclonal antibody (Catalog # A01716-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNGR1 at approximately 95KD. The expected band size for IFNGR1 is at 54KD.
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