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Immunohistochemical staining of human spleen shows strong membranous positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong membranous positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong membranous positivity in cells in red pulp.

Anti-IFNGR1 Antibody

HPA063871
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetIFNGR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IFNGR1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3459
  • Target name
    IFNGR1
  • Target description
    interferon gamma receptor 1
  • Target synonyms
    antiviral protein, type 2; AVP, type 2; CD119; CD119 antigen; CDw119; IFN-gamma receptor 1; IFN-gamma-R1; IFN-gamma-R-alpha; IFNGR; IMD27A; IMD27B; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15260
  • Protein Name
    Interferon gamma receptor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IFNGR1 using anti-IFNGR1 antibody (A01716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HEPG2 whole cell lysates, lane 2: SKOV3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNGR1 antigen affinity purified polyclonal antibody (Catalog # A01716-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNGR1 at approximately 95KD. The expected band size for IFNGR1 is at 54KD.
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