CD125 (IL-5RA): IL-5 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

BPS Bioscience

CD125 (IL-5RA): IL-5 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

23

Research Use Only

Biotin

Interleukin-5

The CD125 (IL-5RA): IL-5 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between CD125 (also known as IL-5RA or Interleukin 5 receptor subunit alpha) and IL-5 protein for screening and profiling applications. This kit comes with enough purified CD125 (amino acids 21-342) and biotinylated IL-5 (amino acids 20-134(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. CD125 (IL-5RA): IL-5 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with CD125 protein. After coating and blocking, biotinylated IL-5 is added in an optimized assay buffer. Next, unbound biotinylated IL-5 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-5 binding to CD125. Need us to run inhibitor screens or profile your compounds that block the binding of IL-5 to IL-5RA? Check out our Immunotherapy Biochemical Screening Services.

41116100

Human