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ProductsBack/CD134 antibody [OX-40]

CD134 antibody [OX-40]

GTX42439
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetTnfrsf4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD134 antibody [OX-40]
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 1/10-1/50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-40
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25572
  • Target name
    Tnfrsf4
  • Target description
    TNF receptor superfamily member 4
  • Target synonyms
    Ox40, Txgp1, tumor necrosis factor receptor superfamily member 4, MRC OX40, OX40 antigen, OX40L receptor, Tax-transcriptionally activated glycoprotein 1, tumor necrosis factor (ligand) superfamily member 4, tumor necrosis factor receptor superfamily, member 4, tumor necrosis factor superfamily, member 4
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP15725
  • Protein Name
    Tumor necrosis factor receptor superfamily member 4
  • Scientific Description
    membrane-bound receptor, binds adaptor proteins Traf2 and Traf5; member of the tumor necrosis factor (TNF)-receptor superfamily [RGD, Feb 2006]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Snelgrove RJ, Cornere MM, Edwards L, et al. OX40 ligand fusion protein delivered simultaneously with the BCG vaccine provides superior protection against murine Mycobacterium tuberculosis infection. J Infect Dis. 2012,205(6):975-83. doi: 10.1093/infdis/jir868
    Read this paper
  • Thaunat O, Graff-Dubois S, Brouard S, et al. Immune responses elicited in tertiary lymphoid tissues display distinctive features. PLoS One. 2010,5(6):e11398. doi: 10.1371/journal.pone.0011398
    Read this paper
  • Volovitz I, Marmor Y, Mor F, et al. T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells. J Autoimmun. 2010,35(2):135-44. doi: 10.1016/j.jaut.2010.05.003
    Read this paper
  • Albillos A, Nieto M, Ubeda M, et al. The biological response modifier AM3 attenuates the inflammatory cell response and hepatic fibrosis in rats with biliary cirrhosis. Gut. 2010,59(7):943-52. doi: 10.1136/gut.2008.168831
    Read this paper
  • Giorgini A, Noble A. Blockade of chronic graft-versus-host disease by alloantigen-induced CD4+CD25+Foxp3+ regulatory T cells in nonlymphopenic hosts. J Leukoc Biol. 2007,82(5):1053-61.
    Read this paper
  • Cousins L, Graham M, Tooze R, et al. Eosinophilic bowel disease controlled by the BB rat-derived lymphopenia/Gimap5 gene. Gastroenterology. 2006,131(5):1475-85.
    Read this paper
  • Stephens LA, Barclay AN, Mason D. Phenotypic characterization of regulatory CD4+CD25+ T cells in rats. Int Immunol. 2004,16(2):365-75.
    Read this paper
  • Humphreys IR, Walzl G, Edwards L, et al. A critical role for OX40 in T cell-mediated immunopathology during lung viral infection. J Exp Med. 2003,198(8):1237-42.
    Read this paper
  • Lavelle EC, McNeela E, Armstrong ME, et al. Cholera toxin promotes the induction of regulatory T cells specific for bystander antigens by modulating dendritic cell activation. J Immunol. 2003,171(5):2384-92.
    Read this paper
  • Walker LS, Gulbranson-Judge A, Flynn S, et al. Compromised OX40 function in CD28-deficient mice is linked with failure to develop CXC chemokine receptor 5-positive CD4 cells and germinal centers. J Exp Med. 1999,190(8):1115-22.
    Read this paper

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Figure 1. Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
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