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CD14 Antibody (clone Sa14-2)

LS-C139900
LifeSpan BioSciences
ApplicationsFunctional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetCD14
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD14 Antibody (clone Sa14-2)
  • Delivery Days Customer
    23
  • Application Supplier Note
    For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The recommended use of this reagent is 10 ul per 250000 cells in a 100 ul total staining volume.. Flo, Func, IP, WB For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The recommended use of this reagent is 10 ul per 250000 cells in a 100 ul total staining volume.
  • Applications
    Functional Assay, Flow Cytometry, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Sa14-2
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID929
  • Target name
    CD14
  • Target description
    CD14 molecule
  • Target synonyms
    monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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