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ProductsBack/Mouse anti Human CD14, conjugated with FITC

Mouse anti Human CD14, conjugated with FITC

Research Use Only
0142
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD14
Price on request
100 tests
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD14, conjugated with FITC
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FWKW
  • Conjugate
    FITC
  • Gene ID929
  • Target name
    CD14
  • Target description
    CD14 molecule
  • Target synonyms
    monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP08571
  • Protein Name
    Monocyte differentiation antigen CD14
  • Scientific Description
    CD14 FITC
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • 1. Immunocytochemical investigation of normal and chronic lymphocytic leukaemia lymphocytes reveals unexpectedly frequent reactivity with some myelomonocytic associated antibodies. Woessner S; Florensa L; Besses C; Vila RM; Galles C; Palou L; Sabrafen JS Leuk Res 1992;16(5):505-10 2. Effect of cytokines and lipopolysaccharide on CD14 antigen expression in human monocytes and macrophages. Landmann R; Ludwig C; Obrist R; Obrecht JP J Cell Biochem 1991 Dec;47(4):317-29 3. Surface markers in adult acute myeloblastic leukemia: correlation of CD19+, CD34+ and CD14+/DR--phenotypes with shorter survival. Groupe d'Etude Immunologique des Leucemies (GEIL). Solary E; Casasnovas RO; Campos L; Bene MC; Faure G; Maingon P; Falkenrodt A. Leukemia 1992 May;6(5):393-9 4. In situ hybridization of interleukin-1 in CD14-positive cells in rheumatoid arthritis. Wood NC; Dickens E; Symons JA; Duff GW Clin Immunol Immunopathol 1992 Mar;62(3):295-300 5. Expression of CD54, CD58, CD14, and HLA-DR on macrophages and macrophage-derived accessory cells and their accessory capacity. Ocklind G; Friedrichs D; Peters JH Immunol Lett 1992 Feb 15;31(3):253-8 6. IL-4 decreases the expression of the monocyte differentiation marker CD14, paralleled by an increasing accessory potency. Ruppert J; Friedrichs D; Xu H; Peters JH Immunobiology 1991 Aug;182(5):449-64