Mouse anti Human CD14, conjugated with FITC

Nordic-MUbio

Mouse anti Human CD14, conjugated with FITC

7

PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

FWKW

FITC

CD14

CD14 molecule

monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein

Mouse

IgG2b

Monocyte differentiation antigen CD14

CD14 FITC

See expiration date on vial

Human

2°C to 8°C

12352203