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ProductsBack/CD144 / CDH5 / VE Cadherin Antibody (clone GF1)

CD144 / CDH5 / VE Cadherin Antibody (clone GF1)

LS-C190059
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDH5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD144 / CDH5 / VE Cadherin Antibody (clone GF1)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo, ICC (1:500), IHC, IHC-P (1:10 - 1:20), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GF1
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1003
  • Target name
    CDH5
  • Target description
    cadherin 5
  • Target synonyms
    7B4; 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; VE-cadherin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VE Cadherin antigen affinity purified polyclonal antibody (Catalog # A02632-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VE Cadherin at approximately 120 kDa. The expected band size for VE Cadherin is at 88 kDa.
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