![ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84801 CD147 antibody [9H5]. ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84801 CD147 antibody [9H5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_1235_ICCIF_w_23061420_352.webp)
ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84801 CD147 antibody [9H5].
CD147 antibody [9H5]
GTX84801
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBSG
Overview
- SupplierGeneTex
- Product NameCD147 antibody [9H5] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:1000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9H5
- Concentration0.64 mg/ml
- ConjugateUnconjugated
- Gene ID682
- Target nameBSG
- Target descriptionbasigin (Ok blood group)
- Target synonyms5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF, basigin, OK blood group antigen, collagenase stimulatory factor, extracellular matrix metalloproteinase inducer, hepatoma-associated antigen, leukocyte activation antigen M6, tumor cell-derived collagenase stimulatory factor
- HostMouse
- IsotypeIgG2b
- Protein IDP35613
- Protein NameBasigin
- Scientific DescriptionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ICC/IF analysis of HT29 cells using GTX84801 CD147 antibody [9H5]. ICC/IF analysis of HT29 cells using GTX84801 CD147 antibody [9H5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_1236_ICCIF_w_23061420_505.webp)
![IHC-P analysis of human kidney tissue using GTX84801 CD147 antibody [9H5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of human kidney tissue using GTX84801 CD147 antibody [9H5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_3142_IHC-P_w_23061420_412.webp)
![IHC-P analysis of human prostate tissue using GTX84801 CD147 antibody [9H5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of human prostate tissue using GTX84801 CD147 antibody [9H5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_3143_IHC-P_w_23061420_633.webp)
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84801 CD147 antibody [9H5]. Loading : 10 ug per lane Dilution : 1:500 WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84801 CD147 antibody [9H5]. Loading : 10 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_4556_WB_w_23061420_454.webp)
![WB analysis of wild type and CD147 knock out 293T cell lysate(10microg per lane) using GTX84801 CD147 antibody [9H5]. Dilution : 1:2000 WB analysis of wild type and CD147 knock out 293T cell lysate(10microg per lane) using GTX84801 CD147 antibody [9H5]. Dilution : 1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_4635_20181228_WB_w_23061420_262.webp)
![WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84801 CD147 antibody [9H5]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84801 CD147 antibody [9H5]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_4636_WB_w_23061420_342.webp)
![WB analysis of various cell lines using GTX84801 CD147 antibody [9H5]. Loading : 35 ug per lane WB analysis of various cell lines using GTX84801 CD147 antibody [9H5]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_4637_WB_w_23061420_682.webp)
![FACS analysis of HEK293T cells transfected with either CD147 plasmid(Red) or empty vector control plasmid(Blue) using GTX84801 CD147 antibody [9H5]. FACS analysis of HEK293T cells transfected with either CD147 plasmid(Red) or empty vector control plasmid(Blue) using GTX84801 CD147 antibody [9H5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84801/GTX84801_554_FACS_w_23061420_756.webp)



![ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84795 CD147 antibody [5D8].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_1229_ICCIF_w_23061420_283.webp)
![ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84798 CD147 antibody [4E4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84798/GTX84798_1231_ICCIF_w_23061420_441.webp)
![ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84799 CD147 antibody [4D3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84799/GTX84799_1233_ICCIF_w_23061420_600.webp)
![ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84800 CD147 antibody [7A7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84800/GTX84800_1234_ICCIF_w_23061420_885.webp)