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ProductsBack/CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI3H1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG. Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG. Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG. Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.

CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI3H1]

CF501163
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBSG
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI3H1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3H1
  • Gene ID682
  • Target name
    BSG
  • Target description
    basigin (Ok blood group)
  • Target synonyms
    5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF, basigin, OK blood group antigen, collagenase stimulatory factor, extracellular matrix metalloproteinase inducer, hepatoma-associated antigen, leukocyte activation antigen M6, tumor cell-derived collagenase stimulatory factor
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP35613
  • Protein Name
    Basigin
  • Scientific Description
    Carrier-free (BSA/glycerol-free) BSG mouse monoclonal antibody, clone OTI3H1 (formerly 3H1)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 2. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (A00248-1). CD147/Emmprin was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CD147/Emmprin Antibody (A00248-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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