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ProductsBack/CD162 antibody [4RA10]

CD162 antibody [4RA10]

GTX13844
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
TargetSelplg
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD162 antibody [4RA10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4RA10
  • Conjugate
    Unconjugated
  • Gene ID20345
  • Target name
    Selplg
  • Target description
    selectin, platelet (p-selectin) ligand
  • Target synonyms
    CD162, Psgl-1, Psgl1, Selp1, Selpl, P-selectin glycoprotein ligand 1, selectin P ligand
  • Host
    Rat
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SELPLG using anti-SELPLG antibody (A03674-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SELPLG antigen affinity purified polyclonal antibody (Catalog # A03674-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SELPLG at approximately 110-120KD. The expected band size for SELPLG is at 110-120KD.
Product group Antibodies
Boster Bio
Anti-PSGL-1/Selplg Antibody Picoband(r)A03674-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetSelplg
  • SizePrice