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Western Blot Positive WB detected in: HL60 whole cell lysate All lanes: Cd163 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 121, 126 kDa Observed band size: 121 kDa
Western Blot Positive WB detected in: HL60 whole cell lysate All lanes: Cd163 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 121, 126 kDa Observed band size: 121 kDa
Western Blot Positive WB detected in: HL60 whole cell lysate All lanes: Cd163 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 121, 126 kDa Observed band size: 121 kDa

Cd163 Antibody

CSB-PA650452LA01MO
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCd163
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Overview

  • Supplier
    Cusabio
  • Product Name
    Cd163 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID93671
  • Target name
    Cd163
  • Target description
    CD163 antigen
  • Target synonyms
    CD163v2; CD163v3; scavenger receptor cysteine-rich type 1 protein M130
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ2VLH6
  • Protein Name
    Scavenger receptor cysteine-rich type 1 protein M130
  • Scientific Description
    Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd163 using anti-Cd163 antibody (A00812-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd163 antigen affinity purified polyclonal antibody (Catalog # A00812-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd163 at approximately 125 kDa. The expected band size for Cd163 is at 125 kDa.
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ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCd163
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