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ProductsBack/CD19 Antibody / B-lymphocyte marker

CD19 Antibody / B-lymphocyte marker

ORB2638153
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
ReactivityHuman
TargetCD19
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CD19 Antibody / B-lymphocyte marker
  • Delivery Days Customer
    10
  • Application Supplier Note
    The stated application concentrations are suggested starting amounts. Variations in protocols, secondaries and substrates may require the anti-CD19 antibody to be titered up or down for optimal performance.
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Applications Supplier
    Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml FACS, IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CVID3/155
  • Conjugate
    Unconjugated
  • Gene ID930
  • Target name
    CD19
  • Target description
    CD19 molecule
  • Target synonyms
    B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15391
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. It is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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