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CD19 antibody [LT19] (APC-Cy7)

GTX28331-15
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD19
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD19 antibody [LT19] (APC-Cy7)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 4 microl reagent / 100 microl of whole blood or 106 cells in a suspension. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LT19
  • Conjugate
    Double Conjugated
  • Gene ID930
  • Target name
    CD19
  • Target description
    CD19 molecule
  • Target synonyms
    B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15391
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Tzeng SJ. The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity. J Vis Exp. 2016,(118). doi: 10.3791/54582
    Read this paper

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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