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ProductsBack/CD19 Monoclonal Antibody
Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 95 kDa
Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 95 kDa
Western Blot Positive WB detected in: Raji whole cell lysate All lanes: CD19 antibody at 1:2000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 95 kDa

CD19 Monoclonal Antibody

CSB-MA004888A1M
Cusabio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD19
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD19 Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4C6G1
  • Conjugate
    Unconjugated
  • Gene ID930
  • Target name
    CD19
  • Target description
    CD19 molecule
  • Target synonyms
    B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP15391
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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