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ProductsBack/CD23 antibody [N3C3]
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE GTX113692 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE GTX113692 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE GTX113692 diluted at 1:1000

CD23 antibody [N3C3]

GTX113692
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetFCER2
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD23 antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2208
  • Target name
    FCER2
  • Target description
    Fc epsilon receptor II
  • Target synonyms
    BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCErII, IGEBF, low affinity immunoglobulin epsilon Fc receptor, C-type lectin domain family 4, member J, CD23 antigen, Fc fragment of IgE receptor II, Fc fragment of IgE, low affinity II, receptor for (CD23), FcepsilonRII, fc-epsilon-RII, immunoglobulin E-binding factor, immunoglobulin epsilon-chain, lymphocyte IgE receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06734
  • Protein Name
    Low affinity immunoglobulin epsilon Fc receptor
  • Scientific Description
    The human leukocyte differentiation antigen CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.[supplied by OMIM]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CD23 using anti-CD23 antibody (A04237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD23 antigen affinity purified polyclonal antibody (Catalog # A04237) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD23 at approximately 36KD. The expected band size for CD23 is at 36KD.
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