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Mouse spleen lysates probed with CD23 Polyclonal Antibody, Unconjugated (bs-2490R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse spleen lysates probed with CD23 Polyclonal Antibody, Unconjugated (bs-2490R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse spleen lysates probed with CD23 Polyclonal Antibody, Unconjugated (bs-2490R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

CD23 Polyclonal Antibody

BS-2490R
Bioss
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetFCER2
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Overview

  • Supplier
    Bioss
  • Product Name
    CD23 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2208
  • Target name
    FCER2
  • Target description
    Fc epsilon receptor II
  • Target synonyms
    BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCErII, IGEBF, low affinity immunoglobulin epsilon Fc receptor, C-type lectin domain family 4, member J, CD23 antigen, Fc fragment of IgE receptor II, Fc fragment of IgE, low affinity II, receptor for (CD23), FcepsilonRII, fc-epsilon-RII, immunoglobulin E-binding factor, immunoglobulin epsilon-chain, lymphocyte IgE receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06734
  • Protein Name
    Low affinity immunoglobulin epsilon Fc receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CD23 using anti-CD23 antibody (A04237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD23 antigen affinity purified polyclonal antibody (Catalog # A04237) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD23 at approximately 36KD. The expected band size for CD23 is at 36KD.
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