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CD27 antibody

10R-6373
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    CD27 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Rat spleen tissue, Rat kidney tissue, Rat liver tissue All lanes: CD27 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
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Figure 1. Western blot analysis of CD27 using anti-CD27 antibody (PB9931). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD27 antigen affinity purified polyclonal antibody (Catalog # PB9931) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD27 at approximately 51 kDa. The expected band size for CD27 is at 29 kDa.
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