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ProductsBack/CD27 Antibody (clone 1E2-A3)

CD27 Antibody (clone 1E2-A3)

LS-C196849
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, Other Application
Product group Antibodies
TargetCD27
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD27 Antibody (clone 1E2-A3)
  • Delivery Days Customer
    23
  • Application Supplier Note
    RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
  • Applications
    Western Blot, ELISA, Other Application
  • Applications Supplier
    ELISA, RNAi, WB RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1E2-A3
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID939
  • Target name
    CD27
  • Target description
    CD27 molecule
  • Target synonyms
    CD27 antigen; CD27L receptor; S152; S152. LPFS2; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; Tp55; tumor necrosis factor receptor superfamily, member 7
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD27 using anti-CD27 antibody (PB9931). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD27 antigen affinity purified polyclonal antibody (Catalog # PB9931) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD27 at approximately 51 kDa. The expected band size for CD27 is at 29 kDa.
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