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ProductsBack/CD28 Antibody (clone 37.51, Azide-free, Low Endotoxin)

CD28 Antibody (clone 37.51, Azide-free, Low Endotoxin)

LS-C812193
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetCD28
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD28 Antibody (clone 37.51, Azide-free, Low Endotoxin)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 microg per 106 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Activ, Block, Flo, IHC, IP Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    37.51
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID940
  • Target name
    CD28
  • Target description
    CD28 molecule
  • Target synonyms
    T-cell-specific surface glycoprotein CD28; Tp44
  • Host
    Hamster
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD28 using anti-CD28 antibody (A00065-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HUT-78 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD28 antigen affinity purified polyclonal antibody (Catalog # A00065-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD28 at approximately 60 kDa. The expected band size for CD28 is at 25 kDa.
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