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Western Blot analysis of K562 cells using CD28 Polyclonal Antibody
Western Blot analysis of K562 cells using CD28 Polyclonal Antibody
Western Blot analysis of K562 cells using CD28 Polyclonal Antibody

CD28 Antibody

CSB-PA005199
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCD28
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD28 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID940
  • Target name
    CD28
  • Target description
    CD28 molecule
  • Target synonyms
    T-cell-specific surface glycoprotein CD28; Tp44
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10747
  • Protein Name
    T-cell-specific surface glycoprotein CD28
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD28 using anti-CD28 antibody (A00065-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HUT-78 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD28 antigen affinity purified polyclonal antibody (Catalog # A00065-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD28 at approximately 60 kDa. The expected band size for CD28 is at 25 kDa.
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