![FACS analysis of Jurkat cells using GTX83004 CD3 zeta antibody [4B10]. Green : CD3 zeta Purple : negative control FACS analysis of Jurkat cells using GTX83004 CD3 zeta antibody [4B10]. Green : CD3 zeta Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX83004/GTX83004_20170912_FACS_w_23061322_502.webp)
FACS analysis of Jurkat cells using GTX83004 CD3 zeta antibody [4B10]. Green : CD3 zeta Purple : negative control
CD3 zeta antibody [4B10]
GTX83004
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD247
Overview
- SupplierGeneTex
- Product NameCD3 zeta antibody [4B10]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4B10
- ConjugateUnconjugated
- Gene ID919
- Target nameCD247
- Target descriptionCD247 molecule
- Target synonymsCD247 antigen, zeta subunit; CD3H; CD3Q; CD3Z; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3-ZETA; CD3zeta chain; IMD25; T3Z; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain; TCRZ
- HostMouse
- IsotypeIgG1
- Protein IDP20963
- Protein NameT-cell surface glycoprotein CD3 zeta chain
- Scientific DescriptionThe protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Mechanism of action of a T cell-dependent bispecific antibody as a breakthrough immunotherapy against refractory colorectal cancer with an oncogenic mutation. Kamakura D et al., 2021 Jan, Cancer Immunol ImmunotherRead more
![ICC/IF analysis of K562 cells using GTX83004 CD3 zeta antibody [4B10]. Green : CD3 zeta Blue: DRAQ5 fluorescent DNA dye ICC/IF analysis of K562 cells using GTX83004 CD3 zeta antibody [4B10]. Green : CD3 zeta Blue: DRAQ5 fluorescent DNA dye](https://www.genetex.com/upload/website/prouct_img/normal/GTX83004/GTX83004_20170912_ICCIF_w_23061322_951.webp)
![IHC-P analysis of human Thymus tissue using GTX83004 CD3 zeta antibody [4B10]. IHC-P analysis of human Thymus tissue using GTX83004 CD3 zeta antibody [4B10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83004/GTX83004_20170912_IHC-P_w_23061322_337.webp)
![WB analysis of CD3 zeta(AA: 52-164)-hIgGFc transfected HEK293 cell lysate using GTX83004 CD3 zeta antibody [4B10]. WB analysis of CD3 zeta(AA: 52-164)-hIgGFc transfected HEK293 cell lysate using GTX83004 CD3 zeta antibody [4B10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83004/GTX83004_20170912_WB_w_23061322_833.webp)

![WB analysis of phosphorylated particular human CD3 zeta mutants using GTX00521 CD3 zeta (phospho Tyr72) antibody [EM-26] and CD3 zeta (phosphoTyr153) [EM-17]. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00521/GTX00521_20191025_AP_001_59_w_23053121_501.webp)


