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Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

CD34 Polyclonal Antibody

BS-0646R
Bioss
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD34
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Overview

  • Supplier
    Bioss
  • Product Name
    CD34 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:500-2000), IHC-P(1:100-500), IHC-F(1:100-500), IP(1:20-50), IF(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    CD34 antigen; hematopoietic progenitor cell antigen CD34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28906
  • Protein Name
    Hematopoietic progenitor cell antigen CD34
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Role of complement 3 in renin generation during the differentiation of mesenchymal stem cells to smooth muscle cells. Chen L et al., 2020 May 1, Am J Physiol Cell Physiol
    Read more
  • Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes. Zhai L et al., 2020 Jan 3, Stem Cell Res Ther
    Read more
  • Transplantation of oral mucosal epithelial cells seeded on decellularized and lyophilized amniotic membrane for the regeneration of injured endometrium. Chen X et al., 2019 Mar 21, Stem Cell Res Ther
    Read more
  • Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte. Jin J et al., 2019 Mar 15, Stem Cell Res Ther
    Read more
  • Gestational exposure to PM2.5 impairs vascularization of the placenta. Yue H et al., 2019 May 15, Sci Total Environ
    Read more
  • MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia-induced cell apoptosis and accelerating angiogenesis. Sha Y et al., 2019 Jun, J Cell Physiol
    Read more
  • Effects of neuritin on the differentiation of bone marrow-derived mesenchymal stem cells into neuron-like cells. Zhu J et al., 2017 Sep, Mol Med Rep
    Read more
  • Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. Long Q et al., 2017 May, Aging Dis
    Read more
  • Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/beta-catenin signaling pathway. Li M et al., 2016 Aug, Oncol Rep
    Read more
  • Hepatitis B Virus Replication in CD34+ Hematopoietic Stem Cells From Umbilical Cord Blood. Huang Y et al., 2016 May 18, Med Sci Monit
    Read more

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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
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