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Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

CD34 Polyclonal Antibody

BS-0646R
Bioss
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD34
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Overview

  • Supplier
    Bioss
  • Product Name
    CD34 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:500-2000), IHC-P(1:100-500), IHC-F(1:100-500), IP(1:20-50), IF(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    hematopoietic progenitor cell antigen CD34, CD34 antigen, CD34 sialomucin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28906
  • Protein Name
    Hematopoietic progenitor cell antigen CD34
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chen L, Fukuda N, Shimizu S, et al. Role of complement 3 in renin generation during the differentiation of mesenchymal stem cells to smooth muscle cells. Am J Physiol Cell Physiol. 2020,318(5):C981-C990. doi: 10.1152/ajpcell.00461.2019
    Read this paper
  • Zhai L, Liu Y, Zhao W, et al. Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes. Stem Cell Res Ther. 2020,11(1):10. doi: 10.1186/s13287-019-1527-z
    Read this paper
  • Chen X, Sun J, Li X, et al. Transplantation of oral mucosal epithelial cells seeded on decellularized and lyophilized amniotic membrane for the regeneration of injured endometrium. Stem Cell Res Ther. 2019,10(1):107. doi: 10.1186/s13287-019-1179-z
    Read this paper
  • Jin J, Shi Y, Gong J, et al. Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte. Stem Cell Res Ther. 2019,10(1):95. doi: 10.1186/s13287-019-1177-1
    Read this paper
  • Yue H, Ji X, Zhang Y, et al. Gestational exposure to PM(2.5) impairs vascularization of the placenta. Sci Total Environ. 2019,665:153-161. doi: 10.1016/j.scitotenv.2019.02.101
    Read this paper
  • Sha Y, Yang L, Lv Y. MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia-induced cell apoptosis and accelerating angiogenesis. J Cell Physiol. 2019,234(6):8846-8861. doi: 10.1002/jcp.27546
    Read this paper
  • Zhu J, Meng P, Wang Q, et al. Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells. Mol Med Rep. 2017,16(3):3201-3207. doi: 10.3892/mmr.2017.6987
    Read this paper
  • Long Q, Luo Q, Wang K, et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. Aging Dis. 2017,8(3):301-313. doi: 10.14336/AD.2016.1018
    Read this paper
  • Li M, Cai H, Yang Y, et al. Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway. Oncol Rep. 2016,36(2):936-44. doi: 10.3892/or.2016.4853
    Read this paper
  • Huang Y, Yan Q, Fan R, et al. Hepatitis B Virus Replication in CD34+ Hematopoietic Stem Cells From Umbilical Cord Blood. Med Sci Monit. 2016,22:1673-81.
    Read this paper

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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
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