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Analysis of CD4 expression in CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line by flow cytometry.
Analysis of CD4 expression in CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line by flow cytometry.
Expression of Influenza A HA TCR in CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line transduced with TCR lentiviruses.
Expression of Influenza A HA TCR in CD4+ TCR Knockout NFAT-Luciferase Reporter Cell Line confers responsiveness to an anti-CD3 agonist, which induces NFAT-dependent luciferase activity
CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat T Cell Line activation after transduction with Influenza A Virus HA TCR Lentivirus (Clone HA1.7), using A375 cells as Antigen presenting cells (APCs).
Analysis of CD4 expression in CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line by flow cytometry.

CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line

Research Use Only
82319
BPS Bioscience
Category(Cell) Culture
Product group (Cell) Culture
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    This cell line was generated by transducing the TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) with lentiviruses (CD4 Lentivirus, Bioscience #78987) to overexpress human CD4 (NM_000616.5). To achieve knockout of TCR (T Cell Receptor), the TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRalpha/beta chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells that stably expressed firefly luciferase under the control of NFAT response elements. TCRalpha/beta knockout in the TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line was confirmed by both genomic sequencing and flow cytometry. Expression of CD4 in CD4+ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line was confirmed by flow cytometry. The cell line does not respond to anti-CD3 agonist antibodies, as opposed to the parental NFAT-Luciferase Reporter Jurkat Cell Line (BPS Bioscience #60621). This cell line has been functionally validated by activation of a transgenic Influenza A Virus HA TCR by the cognate Influenza HA H3 Peptide and MHCII-expressing Antigen Presenting Cells.
  • Storage Instruction
    Liquid Nitrogen
  • UNSPSC
    12352207