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CD40 (H-10) Alexa Fluor(r) 546

SC-13128AF546
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCD40
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200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    CD40 (H-10) Alexa Fluor(r) 546
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 546
  • Gene ID958
  • Target name
    CD40
  • Target description
    CD40 molecule
  • Target synonyms
    Bp50, CDW40, TNFRSF5, p50, tumor necrosis factor receptor superfamily member 5, B cell surface antigen CD40, B cell-associated molecule, CD40 molecule, TNF receptor superfamily member 5, CD40L receptor
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10051) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 43 kDa. The expected band size for CD40/TNFRSF5 is at 30 kDa.
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