ICC/IF analysis of NIH-3T3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC
![ICC/IF analysis of BAF-3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15834/GTX15834_335_ICC-IF_w_23060620_515.webp "ICC/IF analysis of BAF-3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15834/GTX15834_336_ICC-IF_w_23060620_434.webp "ICC/IF analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC")
![FACS analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX15834/GTX15834_123_FACS_w_23060620_200.webp "FACS analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
ICC/IF analysis of NIH-3T3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC
CD44 antibody [1M7.8.1]
GTX15834
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCd44
Overview
- SupplierGeneTex
- Product NameCD44 antibody [1M7.8.1]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:10-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1M7.8.1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID12505
- Target nameCd44
- Target descriptionCD44 antigen
- Target synonymsHERMES, Ly-24, Pgp-1, CD44 antigen, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, PGP-I, extracellular matrix receptor III, hermes antigen, hyaluronate receptor, lymphocyte antigen 24, phagocytic glycoprotein 1, phagocytic glycoprotein I
- HostRat
- IsotypeIgG2b
- Protein IDP15379
- Protein NameCD44 antigen
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Kamioka Y, Takakura K, Sumiyama K, et al. Intravital Förster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli. Cancer Sci. 2017,108(2):226-235. doi: 10.1111/cas.13132Read this paper
Datasheet
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